Thomas Tuchel will have a 27-man squad against Japan with eight of his players leaving the England camp.
Tuchel initially selected 35 Three Lions stars for the friendlies against Uruguay and Japan at Wembley Stadium.
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He revealed that the squad would be split into two groups, with 11 of the selections arriving after the 1-1 draw with Uruguay on Friday.
But now the squad will have a different look for the rest of the break, with eight players departing the England camp.
Squad update
Aaron Ramsdale, Fikayo Tomori and Dominic Calvert-Lewin are among the departures ahead of the match against Japan on Tuesday night, live on eSPORTY.
John Stones is on his way back to Manchester City for assessment after picking up an injury against Uruguay.
“He’s obviously very upset and at the moment he gets my full support because it helps him more than putting more pressure,” Tuchel said of Stones.
He added: “I think he’s a world-class player. But he felt again, I think the calf. We were then very careful and said we don’t take any risk.
“We did a reintegration, he felt it again in the last training. So no risk at the moment.
“But he has a lot of credit in the bank with me. So let’s hope that he gets fit because it’s most important that he finds his confidence.”
Adam Wharton has also picked up a knock and will return to Crystal Palace.
Noni Madueke will follow suit and go back to Arsenal, with Bukayo Saka and Declan Rice joining him for medical assessment.
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Madueke left Wembley in a leg brace after picking he picked up a knock in the first half against Uruguay.
Speaking of his concern on Madueke, Tuchel remarked: “I’m worried, of course, because I think he was a difference-maker today. He started well, he was full of energy, he was ready to go.
“And I think against a team like Uruguay, who relies on one-against-ones, to isolate them there and to go in one-against-ones from every minute out there, the spaces open up and he could have been a key player for us, which he is.
“Very sad and upset and hopefully it’s not a big injury.”
Saka and Rice arrived after the Uruguay match with nine other players as part of the squad split.
Dean Henderson, Nico O’Reilly, Ezri Konsa, Marc Guehi, Dan Burn, Elliot Anderson, Morgan Rogers, Anthony Gordon and Harry Kane were also part of the group.
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Tuesday’s match against Japan will be the final opportunity for players to show Tuchel why they should go to the World Cup.
England must submit their squad by May 30, with friendlies against New Zealand and Costa Rica coming in the following days.
They then play Croatia in their first Group L match on June 17.
